Effect of tetramethylpyrazine on biological properties of tissue-engineered acellular nerve allografts

doi:10.3969/j.issn.2095-4344.2015.03.008

Abstract

Abstract:

BACKGROUND: Increasing studies have shown that tetramethylpyrazine can play certain immunosuppressive effects on cells and allogeneic nerves, reduce the infiltration of inflammatory cells, and reduce the body’s immune rejection to nerve allografts.

OBJECTIVE: To observe the effect of tetramethylpyrazine on the biological characteristics of tissue-engineered nerve bridging materials.

METHODS: Acellular sciatic nerves were extracted from Wistar rats using chemical method to construct gene-modified nerve allografts that were cultured in complete medium containing 200 mg/L tetramethylpyrazine or not for 1 week to observe the cell distribution and structure. Twenty-four healthy adult male Sprague-Dawley rats were enrolled and randomly divided into four groups: in nerve xenograft group, sciatic nerve segments from Wistar rats cultured at 4 ℃ for 1 week were implanted subcutaneously into the back of Sprague-Dawley rats; in control group, tissue-engineered nerve segments cultured in the complete medium were implanted; in experimental group, tissue-engineered nerve segments cultured in the complete medium containing 200 mg/L tetramethylpyrazine were implanted; in nerve allograft group, nerve allografts from Sprague-Dawley rats were implanted. After 7 days, nerve segments were taken out for hematoxylin-eosin staining under light microscope, and the number of CD4⁺ and CD8⁺ lymphocytes was counted.

RESULTS AND CONCLUSION: The acellular nerve segments with no cellular constituents had intact epineurium and basement membrane. After injection of nerve cells, there were more cellular components that distributed unevenly beneath the epineurium and between the perineurium. After 1 week of culture, more nerve cells proliferated and differentiated in the complete medium containing 200 mg/L tetramethylpyrazine or not, especially in the complete medium containing 200 mg/L tetramethylpyrazine. Under the light microscope, the degree of lymphocyte infiltration ranged from more to less: nerve xenograft group, control group, experimental group, nerve allograft group. These findings indicate that tetramethylpyrazine can reduce tissue-engineered nerve immunogenicity, but cannot destroy the three-dimensional structure of tissue-engineered nerves.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Tissue Engineering, Drugs, Chinese Herbal, Neural Stem Cells

CLC Number:

R318

Xiang Fei-fan, Yang Yun-kang. Effect of tetramethylpyrazine on biological properties of tissue-engineered acellular nerve allografts[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(3): 370-376.

Figures/Tables 9

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